By G. Antranikian, G. Antranikian, M. Ciaramella, M.S. da Costa, E.A. Galinski, R. Ladenstein, S. Maloney, V.T. Marteinsson, M. Moracci, R. Müller, J. van der Oost, F.M. Pisani, D. Prieur, N.J. Russell, M. Rossi, H. Santos, R. Sharp, W.M. de Vos
During this unique factor assessment articles summarize the main amazing gains of microorganisms which may live on less than severe stipulations. those microorganisms are tailored to residing at a hundred° C in volcanic springs, at low temperatures within the chilly polar seas, at excessive strain within the deep sea, at very high and low pH values (pH 0-1 or pH 10-11), or at very excessive salt concentrations (35%). the newest findings at the molecular biology and the protein constitution of those unique organisms are provided. furthermore, the capability purposes of extremophiles are reviewed, together with the construction of enzymes, suitable solutes and using those extremophiles within the degradation of xenobiotics.
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Extra info for Biotechnology of Extremophiles
Proteolytic Enzymes . . . . . . . . . . . . . . . . . . . . DNA-Polymerases . . . . . . . . . . . . . . . . . . . . . 3 4 ................................................ References . . . . . . . . . . . . . . . . . . . . . . . . d. 185 Joule) Kelvin /~ngstr6m (1 ~k = 10 -10 m) Number of atoms Number of buried atoms Calculated surface area Observed surface area Distance Root mean square distance Alpha carbon atom Difference Free energy Enthalpy Entropy Heat Capacity abbreviations are explained in the text Keywords.
2 Proteins are Only Marginally Stable, but the Forces Holding Them Together are Highly Cooperative Referring now to the first question raised above, several well known facts of protein stability must be taken into account: the free energy of stabilization of a globular protein - it may be mesophilic or thermophilic - is quite small. It is not larger than about 7-15 kcal/mol [5, 10] which is equivalent to the energy which results from the formation of a few hydrogen bonds, ion pairs or hydrophobic interactions.
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Biotechnology of Extremophiles by G. Antranikian, G. Antranikian, M. Ciaramella, M.S. da Costa, E.A. Galinski, R. Ladenstein, S. Maloney, V.T. Marteinsson, M. Moracci, R. Müller, J. van der Oost, F.M. Pisani, D. Prieur, N.J. Russell, M. Rossi, H. Santos, R. Sharp, W.M. de Vos