By Zhihong Xu (auth.), Zhihong Xu, Jiayang Li, Yongbiao Xue, Weicai Yang (eds.)
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Additional resources for Biotechnology and Sustainable Agriculture 2006 and Beyond: Proceedings of the 11th IAPTC&B Congress, August 31-18, 2006 Beijing, China
Other more specialist applications include the production of high value compounds by somatic embryos grown in bioreactors. Probably the most impressive example of this is the production of the B subunit of Escherichia coli heat-labile toxin (LTB), a potent mucosal immunogen and immunoadjuvant for co-administered antigens. In order to produce a large scale of LTB for the development of an edible vaccine, transgenic somatic embryos of Siberian ginseng were cultured in a 130 L airlift type bioreactor.
Bakker H, Bardor M, Molthoff JW, Gomord V, Elbers I, Stevens LH, Jordi W, Lommen A, Faye L, Lerouge P, Bosch D (2001) Galactose-extended glycans of antibodies produced by transgenic plants. Proc Natl Acad Sci USA 98: 2899–2904. Blixt O, Allin K, Pereira L, Datta A, Paulson JC (2002) Efficient chemoenzymatic synthesis of O-linked sialyl oligosaccharides. J Am Chem Soc 124: 5739–5746. CPMP (2002) Points to consider on quality aspects of medicinal products containing active substances produced by stable transgene expression in higher plants (CPMP/BWP/764/02).
2006). , see Platis and Labrou, 2006). In industrial processing, robust and inexpensive chromatography media are used in the initial steps, accepting that there will be some loss of selectivity and resolution (Bai and Glatz, 2003; Menkhaus and Glatz, 2005). , His6 and Ni-NTA resin) which are highly selective initial capturing methods. 28 R. Fischer et al. One of the greatest uncertainties surrounding the use of plants for the production of pharmaceuticals is the regulatory landscape. While plants are grown in glasshouses and in enclosed bioreactors, the production of pharmaceuticals is regulated in the same way for other production systems, and comes under the authority of the FDA and equivalent agencies in other parts of the world.
Biotechnology and Sustainable Agriculture 2006 and Beyond: Proceedings of the 11th IAPTC&B Congress, August 31-18, 2006 Beijing, China by Zhihong Xu (auth.), Zhihong Xu, Jiayang Li, Yongbiao Xue, Weicai Yang (eds.)